Dose-dependent neutralizing-antibody responses to vaccinia.
2004 Feb 01
Journal Article
Authors:
Belshe, R.B.;
Newman, F.K.;
Frey, S.E.;
Couch, R.B.;
Treanor, J.J.;
Tacket, C.O.;
Yan, L.
Secondary:
J Infect Dis
Volume:
189
Pagination:
493-7
Issue:
3
PMID:
14745707
DOI:
10.1086/380906
Keywords:
Adolescent; Adult; Antibodies, Viral; Blister; Dose-Response Relationship, Immunologic; Female; Fever; Humans; Male; Neutralization Tests; Single-Blind Method; Smallpox Vaccine; United States; Vaccination; Vaccinia; Viral Plaque Assay
Abstract:
To evaluate the humoral immune responses to smallpox-vaccine stocks currently available in the United States (Dryvax; Wyeth) and to generate data for comparison of responses to newly produced lots of smallpox vaccine, we evaluated dose-response effects, using undiluted and diluted smallpox vaccine. At 28 and 56 days after vaccination, serum samples were obtained from vaccinated subjects (N=674) who had participated in a randomized, single-blinded trial of an undiluted or a 1 : 5 or 1 : 10 dilution of smallpox vaccine and who subsequently were tested for plaque-reduction neutralizing-antibody titer. All subjects who developed a vesicle after vaccination also developed neutralizing antibodies by day 28. Subjects given either a 1 : 5 or 1 : 10 dilution of vaccinia had significantly higher neutralizing-antibody titers than did subjects given undiluted vaccine. Larger lesion size and fever after vaccination were associated with significantly higher neutralizing-antibody titers after vaccination.
